NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training Course

This course provides comprehensive, hands-on instruction aligned with industry standards to prepare participants for the NSF 49 written and practical exams. The NSF advanced validation training course offers in-depth training on BSC design, construction, functions, installation requirements, and safe operation practices.

Participants perform advanced certification procedures through practical exercises such as site assessments and airflow testing. Training includes all major NSF primary and secondary tests, including inflow and downflow velocity testing, airflow pattern testing, HEPA filter leak testing, vibration, lighting, noise level tests, and mock gas decontamination. Calculating airflow for Type B2 cabinets is also covered.

Please note: NSF exam registration requires a separate application and fee, and must be arranged directly with NSF at least 30 days in advance. EAB Training does not guarantee exam participation.
Recommendations: Click here for more info | Classroom Instruction Time: 15 Hours | Hands-On Instruction Time: 20 Hours | eLearning: Online LMS Resources for six months | NSF CE Credits: 5 Credits | Examination Time: Determined by the NSF proctor

What's Included in the Course?

  • 5 Days of Instruction and 2 Days of Testing
  • Resource Materials:
  • EAB Training provides the current version of the NSF/ANSI 49 Biosafety Cabinetry: Design, Construction, Performance, and Field Certification Standard
  • EAB Training provides a version of the NSF Policies for the Enhanced Accreditation Programs.
  • EAB Training Course Workbook and Materials
  • Access to the online EAB Learning Management System (LMS) for six months
  • Completion certificate and NSF continuing education credits
  • Instrumentation Instruction
  • Breakfast and Lunch on Instructional Days

What Should I Bring to EAB Training?

  • Computer or tablet with internet capabilities to access the EAB Learning Management System (LMS).
  • A calculator that is not on a computer, tablet, or smart device.
  • Your personal copy of the NSF/ANSI 49 Biosafety Cabinetry: Design, Construction, Performance, and Field Certification Standard if you have one.

What About Meals and Snacks?

Breakfast, lunch, and snacks are included Monday – Friday for EAB Training Students. Please let us know if you have any dietary restrictions by emailing training@eabcoinc.com.

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NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training

This course covers the following topics:
  • Overview of Biological Safety Cabinets
  • Introduction to Data Sheets and Certification
  • Instructional and practical training on the following:
  • Downflow Velocity Test
  • Inflow Velocity Test – DIM Method
  • Inflow Velocity Test – Constricted Access Opening Method
  • Calculating Inflow/Downflow Velocity from Volume – Type B2 Cabinet
  • HEPA Filter Leak Test – Type A Cabinet (Scan Method)
  • Exhaust HEPA Filter Leak Test – Type B Cabinet (Probe Method)
  • Vibration Test
  • Lighting Intensity Test
  • Noise Level Test
  • Site Installation Assessment Test
  • Airflow Pattern Test
  • Cabinet Gas Decontamination (Mock)
Recommendations: Click here for more info Classroom Instruction Time: 15 Hours Hands-On Instruction Time: 15 Hours eLearning: Online LMS Resources for six months NSF CE Credits: 5 credits Examination Time: Determined by the NSF proctor

Course Date:
October 19, 2026 – October 25, 2026

There is a separate application process and fee for the NSF exam, and EAB Training does not guarantee your participation in the NSF exam. NSF exam arrangements are required at least 30 days in advance with NSF directly.

Daily Agenda

Agenda based on the NSF 49 Workflow Order of Operations

  • Downflow velocity
  • Inflow velocity (primary, direct inflow measurement)
  • Inflow velocity (secondary constricted access)
  • Calculating downflow/inflow velocity from volume (Type B2)
  • HEPA filter leak scan
  • HEPA filter leak gross probe
  • Site installation assessment
  • Smoke patterns
  • Lighting intensity
  • Vibration test
  • Noise level test
  • Decontamination test

Day One

7:30 am
Breakfast Welcome Slide Deck and Introductions NSF 49 Introduction BSC Cabinet Classification Types and Airflow Patterns Biological Safety Levels (BSL) Overview HEPA filter types Design, Construction, and Memorandums Sample NSF Data Sheet Overview Common Failure Scenario Introduction Instrumentation and Equipment Overview Apparatus Testing Equipment and Calibration Overview
12:00 pm
Lunch
1:00 pm
Downflow Velocity Test – Discussion and Data Sheet Detail Laboratory Area Tour Downflow Velocity Test – Lab Practice Practical Inflow Velocity Test (DIM Method) – Discussion and Data Sheet Detail Inflow Velocity Test (DIM Method) – Lab Practice Practical Inflow Velocity Test (Constricted Method) – Discussion and Data Sheet Detail Inflow Velocity Test (Constricted Method) – Lab Practice Practical Day 1 Wrap-up and Q&A Session
5:00 pm
Day 1 Dismissal

Day Two

7:30 am
Breakfast / Review Day 1 / Overview of Day 2 Objectives Calculating Downflow / Inflow Velocity From Volume (Type B2)-Discussion and Data Sheet Detail Calculating Downflow / Inflow Velocity From Volume (Type B2) – Lab Practice Practical HEPA Filter Leak Test – Type A (Scan Method) – Discussion and Data Sheet Detail HEPA Filter Leak Test – Type A (Scan Method)
12:00 pm
Lunch
1:00 pm
Exhaust HEPA Filter Leak Test-Type B (Probe Method)-Discussion and Data Sheet Detail Exhaust HEPA Filter Leak Test – Type B (Probe Method) Site Installation Assessment Test – Discussion and Data Sheet Detail Site Installation Assessment Test – Lab Practice Practical Airflow Smoke Pattern Testing – Discussion and Data Sheet Detail Airflow Smoke Pattern Testing – Lab Practice Practical Day 2 Wrap-up and Q&A Session
5:00 pm
Day 2 Dismissal

Day Three

7:30 am
Breakfast / Review Day 2 / Overview of Day 3 Objectives Light Intensity Test – Discussion and Data Sheet Detail Light Intensity Test – Lab Practice Practical Vibration Testing – Discussion and Data Sheet Detail Vibration Testing – Lab Practice Practical Noise Level Testing – Discussion and Data Sheet Detail Noise Level Testing – Lab Practice Practical
12:00 pm
Lunch
1:00 pm
Decontamination Procedures – Discussion and Data Sheet Detail Decontamination Procedures – Mock Lab Practice Practical
5:00 pm
Day 3 Wrap-up and Q&A Session

Day Four

7:30 am
Breakfast / Review Day 3 / Overview of Day 4 Objectives
Mock Practical Exams (Lunch will be provided)
1. Downflow Velocity
2. Inflow Velocity Test – DIM Method
3. Inflow Velocity Test – Constricted Method
4. Calculating Downflow / Inflow velocity From Volume (Type B2)
5. HEPA Filter Leak Test – Scan Method (Type A)
6. Exhaust HEPA Filter Leak Test – Probe Method
7. Site Installation Assessment Test
8. Airflow Smoke Patterns Test
9. Lighting Intensity Test
10. Vibration Test
11. Noise Level Test
Day 4 Wrap-up and Q&A Session
5:00 pm
Day 4 Dismissal

Day Five

7:30 am
Breakfast / Review Day 4 / Overview of Day 5 Objectives
12:00 pm
Lunch & Study
12:30 pm
Prepare Testing Room (If applicable)
1:00 pm
NSF Proctored Written Exam (If applicable)
Review for Next Day NSF Proctored Practical Exam & Exit Surveys

Day Six

7:30 am
EAB Testing Site – Students & Proctors are responsible for their own food and necessities Group 1 NSF Proctored Practical Exam (If applicable) Day 6 Wrap-up and Clean-up
6:30 pm
Day 6 Practical Testing Day Dismissal

Day Seven

7:30 am
EAB Testing Site – Students & Proctors are responsible for their own food and necessities
Group 1 NSF Proctored Practical Exam (If applicable)
Day 7 Wrap-up and Clean-up
6:30 pm
Day 7 Practical Testing Day Dismissal

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